DNA PARENT VALIDATION

How to collect and send DNA samples to NZWA.

DNA samples are to be mailed to the NZWA Registrar:  Christine Hartstone, 1 Ohautira Road, RD1, Raglan 3295

To collect a sample of your horse, first make sure the horse is not wet when collecting the sample.

A sample is collected by pulling 30-40 mane and/or tail hairs from a horse. It is important to pull the hairs and confirm that the actual root of the hair is attached. The root of the hair is what contains the genetic material of your horse. Therefore, hairs that break off or cut hairs do not provide an adequate sample of your horse.

The mane hair of a foal can be very fine and not have much of a root for us to work with. When collecting a sample from a young foal, it is often better to collect the hair from the tail of the horse instead of the mane.

Once the hair has been collected, place the sample immediately in a zip-lock bag or paper envelope that is labelled with the horse’s name. Again, it is important that the hair sample is not wet especially when using a zip-lock bag. Moisture and warmth trapped within the bag might give bacteria an opportunity to grow which could damage the DNA.

To be eligible for Registration, a foal may be produced by:
         - Natural service. - If a mare is bred by more than one stallion in a season,
               then DNA testing will be required
.
         - Artificial insemination (AI) – with Fresh semen DNA testing is not required
         - Frozen semen - subject to DNA testing.
         - Embryo Transfer (ET) foals - subject to DNA testing.


STALLIONS

After 1 August 1987 all stallions registered with NZWA must be DNA-typed at the owner’s expense prior to being used for breeding.
An official copy of the DNA type will be filed with NZWA.


EMBRYO TRANSFER*

Embryo transfer is permitted provided that written advice is provided to the President and Registrar including details of the intended sire and dam, and the veterinarian/ professional conducting the embryo transfer. The following are also required:

(a) A service certificate is provided from the Stud Master authenticating that the semen has been collected from the stallion.

(b) The veterinarian carrying out the procedure certificates that the embryo was flushed from the donor mare and the mare was inseminated/naturally served with the semen as above,

(c) It will be necessary to DNA-type the foal and the biological dam before registration is permitted.

The NZWA will register a maximum of 3 resultant foals due to embryo transfer per year per mare.

*Taken from the BREED REGULATIONS (NZWA) updated 2014